Cell Culture Equipment

Cell perfusion and culture is conducted in the Cell Perfusion and Cell Imaging Lab, Mechanical and Industrial Engineering, room 403B. To get access to this lab see our Access and Fees page.

Biological Safety Cabinet

Biological Safety Cabinet

Biological Safety Cabinet

Model:  Thermo Scientific 1300 Series A2

Class: Class II, Type A2

Width: 36″

Work aperture: 8″

Manual

 

Aspirator

Aspirator

Aspirator

Model: Scienceware Vacuum Aspiration Collection System

Volume: 3.8L

Pressure: 0.3 m³/min

Manual

 

Autoclave

Autoclave

Autoclave

Model: Tuttnauer (model 3850)

Maximum temperature: 150 °C

Maximum pressure: 50 PSI

Chamber volume: 65L

Programs: unwrapped instruments, unwrapped delicate instruments, liquid, liquid + cooling

Manual

 

Real Time PCR – 96 or 384 wells

Real-time PCR

Real-time PCR

Model: Bio-Rad CFX96 and CFX384

Temperature range: 0 – 100 °C

Excitation/emission: 450 – 760 nm

Sensitivity: Detects 1 copy of target sequence in human genomic DNA

Dynamic range: 10 orders of magnitude

Scan time: 3 (FAM/SYBR® Green only) – 12 (all channels) seconds

Sample size: 1 – 50 μL (CFX96) or 1 to 30 μL (CFX384)

Software:  CFX manager software

Manuals: preparing a plate, writing a protocol, analysis of resultsgene expression, and full PCR manual

 

Thermal Cycler

Thermal Cycler

Thermal Cycler

Model: Bio-Rad T100 Thermal Cycler

Format: 96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate

Temperature range: 4 – 100 °C

Manual

 

Reverse Osmosis and Deionized Water

DI water system

DI water system

Model: Purlab flex by Elga water suppliers

Quality: reverse osmosis and deionized

Flow rate: up to 2 L per minute

Autovolume: 100 to 500 mL

Manual

 

 

Cell Counter

Automated cell counter

Automated cell counter

Model: Bio-Rad T20

Measurement time: 30 seconds

Cell concentration range: 5 x 10^4–1 x 10^7 cells/mL

Cell diameter: 6 – 50 μm

Sample volume: 10 μL

Manuals: quick guide and full manual

 

Centrifuge

Legend Micro 21R Centrifuge

Legend Micro 21R Centrifuge

Model: Thermo Scientific Sorvall Legend Micro 21R and  Legend X1R

Temperature: -9 – +40 °C

Max Time: 99 min (21R), 99 hr 59 min (X1R)

Max speed: 14,800 rpm (21R), 15,200 rpm (X1R)

Micro 21R manuals:  manualquick instructions

Micro 21R samples: 24 x 1.5 or 2 mL tubes

X1R manuals:  manualquick instructions

X1R samples: 28 x 15mL tubes, 16 x 50 mL tubes, 2 well plates

Freezer

-80 C Freezer

-80 C Freezer

Model: Thermo Scientific Forma 900 Series

Temperature: -80 °C

Storage: 4 inner sequestered areas each 5 x 5 selves, 4 sample boxes deep

Manual

 

 Water Bath

Fisher Scientific water bath.

Fisher Scientific water bath

Model: Fisher Scientific Isotemp

Set Temperture: 37 °C

 

 

 

Scale

Mettler Toledo Analytical Scale

Analytical Scale

Models: Mettler Toledo analytical balance, Denver Instruments balance

Maximum weight: 120 g (analytical balance), 210 g (balance)

 

 

 

Hot Plate

Hot plate with stirrer

Hot plate with stirrer

Model: Fisher Scientific stirring hotplate

Maximum temperature: 540 °C

Maximum stir speed: 1200 rpm

Maximum weight: 25 lbs

Manual

 

pH Meter

pH meter

pH meter

Model: Accumet basic pH meter

Manual

 

Pipette Filler

Serological pipette filler

Serological pipette filler

Model: Scilogex

Pipettes: 1 mL, 5 mL, 10 mL, 25 mL provided

Power: rechargeable battery

 

Micropipette

Micropipettes

Micropipettes

Model: Accumas Smart

Sizes: 0.5 – 2 µL, 2 – 20 µL, 20 – 200 µL and 100 µL – 1 mL

Tips: provided