August 19th, 2013
Dr. David Eddington will visit CCBR on August 27th and present a talk titled “Controlling Oxygen Gradients via Microfluidic Devices for Cells and Tissues in vitro”
For more information on Dr. Eddington’s work visit his website.
For more information about this talk, see the attached flyer.
Tuesday August 27, 2013, 10am-11:30am, The Donnelly Centre, Yip/Friesen Red Seminar Room
My lab has designed, fabricated, and validated a suite of devices to easily and accurately regulate oxygen levels in cellular cultures within standard culture formats such as multiwell plates, Boyden chambers, and 3D cell matrices. Oxygen can be delivered or extracted to the cells via simple diffusion and is rapid due to the microscale distances separating the oxygen microchannels from the cell cultures. This oxygen can also be patterned onto anatomical regions of tissue, in gradients across a substrate, or cyclic profiles of high or low oxygen. In addition, due to the rapid equilibration time, intermittent hypoxia profiles or ischemia reperfusion studies are easy to perform in a format compatible with fluorescence microscopy for real time analysts of cell or tissue function. We are applying these devices with researchers studying topics varying from insulin release from islets under hypoxia, strokes in acute brain slice preparation, wound healing, and hypoxia signaling pathways.