Monday August 19, 2013.
David Juncker visited CCBR and presented his work on microarrays. For more information about this talk visit David’s website.
ABSTRACT: In this talk I will focus on two arraying technologies developed in our lab and their applications. The first is one is the so called snap chip that allows parallel transfer of multiple reagents and was used to service 100 um large spots with droplets of detection antibody for a multiplexed sandwich immunoassays. This platform can be used for realizing the antibody colocalization microarray in a simple manner, and be scaled up to measure multiple proteins simultaneously. The use for time course analysis of breast cancer model in mouse will be discussed, as well as storage of snap chips prior to usage. Next, a novel low coat nanocontact printing method will be presented along with digital nanodot gradients for studying cell Haptotaxis. Using this technique, 7.6 million spots making up 64 DNGs were printed in 5 s. The dynamic range of the gradients was over 4400, matching physiological scales and 100 fold higher than previous approaches. I will discuss our efforts in systematically tuning the affinity between the non-patterned reference surface to the cells to optimize the cell response to the patterned proteins. The response of fibroblasts and myoblasts to RGD-peptide dots and Netrin-1 gradients in will be presented.